Proteins Specified by Herpes Simplex Virus VI. Viral Proteins in the Plasma Membrane
نویسندگان
چکیده
منابع مشابه
Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion.
We are reporting a procedure for the purification of herpes simplex enveloped nucleocapsids (virions), an evaluation of the purification procedure and the results of analyses of the virion proteins by high-resolution acrylamide gel electrophoresis. The data may be summarized as follows. (i) The procedure for the purification of virions consists of careful extraction of cytoplasm to prevent nucl...
متن کاملStructural proteins of herpes simplex virus.
Herpes simplex virus, grown in BHKzI cells, was extensively purified by fluorocarbon treatment, ultracentrifugation, sucrose gradient centrifugation and chromatography on calcium phosphate. The purity of the product was assessed by immunodiffusion, electrophoresis in polyacrylamide gels and removal of added radioactive host material from the virus. Purified virus was disrupted with sodium dodec...
متن کاملComparison of the intranuclear distributions of herpes simplex virus proteins involved in various viral functions.
Herpesviral transcription, DNA synthesis, and capsid assembly occur within the infected cell nucleus. To further define the spatial relationship among these processes, we have examined the intranuclear distributions of viral DNA replication, gene regulatory, and capsid proteins using dual label immunofluorescence and confocal microscopy. We observed that several of the viral DNA replication pro...
متن کاملHerpes simplex virus 1 VP22 regulates translocation of multiple viral and cellular proteins and promotes neurovirulence.
Herpes simplex virus 1 (HSV-1) protein VP22, encoded by the UL49 gene, is a major virion tegument protein. In the present study, we showed that VP22 was required for efficient redistribution of viral proteins VP16, VP26, ICP0, ICP4, and ICP27 and of cellular protein Hsc-70 to the cytoplasm of infected cells. We found that two dileucine motifs in VP22, at amino acids 235 and 236 and amino acids ...
متن کاملInteractions between herpes simplex virus DNA-binding proteins.
Monoclonal antibodies directed against several herpes simplex virus (HSV)-induced DNA-binding proteins were used to investigate protein interactions in HSV-infected cells. Q1 monoclonal antibody, which is specific for the HSV-induced alkaline nuclease, when used in an immunoadsorbant column resulted in the purification of the alkaline nuclease, to which large quantities of the major DNA-binding...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1972
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.9.3.431-439.1972